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KMID : 1059519950390060453
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Journal of the Korean Chemical Society
1995 Volume.39 No. 6 p.453 ~ p.458
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Analysis of Higher Order Structure of 5S rRNA from Pseudomonas alcaligenes by using Pb(Ⅱ) Ion
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Kim Sang-Bumn
Lee Young-Hoon
Park In-Won
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Abstract
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We have applied Pb2+ to the structural analysis of 5S rRNA from Pseudomonas alcaligenes. The mode of Pb2+-induced clevage on 5S rRNA has shown several specific features which may be utilized for the examination of tertiary structure of 5S rRNA: Pb2+ does not attack the stable helical stems; single stranded regions or bulges are attacked in variable susceptibilities depending on the positions of the sequences or the bases on the molecule; unstable helical region d is not attacked at all; only 3' sided strand of unstable helical stem C is weakly attacked, leaving 5' sided strand unattacked. Based on the Pb2+ cleavage properties and the structural analysis of Xanthomonas celebensis 5S rRNA, we have proposed a working hypothesis for the tertiary interactions in 5S rRNA.
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